16th Congress of the International Society for Forensic by Erika Hagelberg (auth.), Professor Dr. med. Angel Carracedo,

By Erika Hagelberg (auth.), Professor Dr. med. Angel Carracedo, Professor Dr. med. Bernd Brinkmann, Professor Dr. med. Walter Bär (eds.)

This booklet presents an summary of up to date examine in forensic genetics ordinarily, and specifically at the software of DNA know-how in forensic casework. a large choice of DNA polymorphisms, in particular STRs, different PCR established polymorphisms, and mt-DNA are studied greatly and new applied sciences and methodologies comparable to capillary electrophoresis, lengthy PCR or MVR strategy are mentioned. inhabitants facts, sequencing facts, and forensic purposes of DNA polymorphisms including statistical standardization and moral difficulties also are lined with contributions by means of the major scientists within the field.

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Japan **Dept of Biological Sciena:s. GraWate School of Science. The Univ of Tokyo. Tokyo 113. Japan ***Dept of Legal Med. Univ ofTsukuba. 1-1-1 Tennodai. Tsukuba305. Japan Introduction The analysis of highly polymorphic regions of mitochondrial DNA is one of the most commonly used methods for personal identification. The recent advances of fluorescent detection in automated DNA sequencing (Smith et al. 1986) has made it possible a rapid analysis of sequence variants without using isotopic labeling.

The grandmother said that she had killed a mouse in the bag. Analysis of the DNA showed that the bloodstains found on the bag were from "mus domesticus" (mouse), the bloodstains from bowl were from "Gallus gallus" (chicken) and the bloodstains from the knife were from "Sus scrofa" (pig). Human identification In the past DNA, extracted from bones or from rootless hair have been very difficult to type. We currently use mitochondrial DNA for exclusion or identification. Two main problems have to be considered : -Contaminations DNA is present in every nook of a molecular biology laboratory and we have to avoid the contamination of the sample by exogenous DNA and amplification products.

Co CD '" l!! :!! , 10 N N G A ~ C N N N ~ .. ,'" ... ... , N N C A .. 2'" 0 Ie T T C C T ~ I T C A T T T T G A C C C C T A Table 2: Distribution of mtDNA sequences in Norwegian Saami populations. SAAMI SAAMI Obs. Anaerson-81 TOTAL:133 . .. ,'" .... '" '"co '" ~ § § § § § § §'" CD '~" ~ HAPLOTYPES I A U (; G A T N It) C C CD ~ ... ~ C C C C C -- t---- C C C C C C I T T T C G G T T T T T T C A T T I t-r-- G T C :-- I---- , T I C 25 PCR products were obtained from all samples. All 201 Saami lacked the 9 bp deletion commonly seen in Asians (Hertzberg et al.

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